Transcription of Genetic Engineering Methods - Oregon State
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Genetic Engineering MethodsOutline Why do it? Research examples: poplar trees Plant gene transfer concepts and Methods Getting genes ready for transfer (recombinant DNA/plasmids) Analysis of transgenic plantsWhy do it? To create new, desired trait not in accessible gene pool To breed faster, more directly Dominant, rare alleles To amplify Genetic diversity for particular genes/traits for breeding To breed with more direction, science basis To use plants as bioproductfactories for industry/medicine To promote biosafety for exotic/unsafe crop (domestication, sterility) In all cases, new traits/diversity feeds into breeding to check yield/stability of whole organism in the field: integrated health testNative gene alteration example.
Genetic Engineering Methods. Outline ... first step in genetic engineering. The cells on the edges of the disk are wounded in the process of cutting so they can receive a new gene from Agrobacterium tumefaciens. Step-by-step view of poplar transformation. Summary of steps in regenerating
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