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Guide to Performing Relative Quantitation of Gene Expression Using Real- time Quantitative PCR. 1. Guide to Performing Relative Quantitation of Gene Expression Using Real- time Quantitative PCR. Table of Contents Section I: Introduction to Real- time PCR and Relative Quantitation of Gene Expression pg 4. 1. Introduction 2. What is Relative Quantitation ? 3. Terms and Acronyms 4. Relative Quantitation of Gene Expression Requires the Quantitation of Two Different Genes (Target and Endogenous Control). 5. Factors Affecting Accurate Real- time PCR Results 6. What is PCR Amplification Efficiency? Section II: RNA Preparation and Reverse Transcription pg 14. 1. Introduction 2. Quantifying Input RNA. 3. Reverse Transcription (RT) for Relative Quantitation of Gene Expression a.
Section VI. Ordering Real Time PCR Reagents pg 42 Section VII. Relative Quantitation of Gene Expression Experimental Design and Analysis pg 43
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