Transcription of HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
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HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
1 HIGH PERFORMANCELIQUID CHROMATOGRAPHY (HPLC)Chapter 28 NOTE: HPLC came about because not allcompounds can be vaporizedand analyzed on a GCSource: Harris, Exploring Chemical Analysis, 2nded. (2001) 2 Summary of MethodMobile phase: LiquidHigh PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) An analytical separation technique that involves the high-pressure flow of a LIQUID through a column that contains the stationary phase: Can be a solid (LSC) or a LIQUID (LLC) A mixture of compounds injected at one end of the column separates as the compounds pass through. Separated compounds are detected electronicallyas they elute at the other end of the with GCGCHPLCB asis of separationInteractionof solutes with the ; solute vapor pressureInteractionof solutes with boththe and timeFast(a few minutes for simple mixtures)Slower than GC (several minutes for a simple mixtureTemperature for separationUsually requires a high temper)
flow of a liquid through a column that contains the stationary phase. Stationary phase: Can be a solid (LSC) or a liquid (LLC) A mixture of compounds injected at one end of the column separates as the compounds pass through. Separated compounds are detected electronically as they elute at the other end of the column. Comparison with GC GC HPLC ...
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