Transcription of Expt 2 Calcium by visible spectroscopy
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Expt 2 Calcium by visible spectroscopy
1 | P a g e CHEM 450 expt . 2: DETERMINATION of Calcium in URINE SAMPLES by visible (Vis) spectroscopy Advanced Preparation: To be completed before coming to the lab. Show calculations to your instructor before preparing any of the solutions below. 100 mL of M Ca2+ from 400 ppm Ca2+ stock solution 200 mL of a x 10-3 M aqueous solution of Arsenazo III (FW g/mole) 1 L of M Imidazole buffer from the hydrochloride salt, adjusted to pH with either M HCl or M NaOH Background Soluble Calcium (as Ca2+ ions) in aqueous samples is usually determined by EDTA titration using Eriochrome Black T as indicator. In very dilute solutions, titration becomes impractical to use due to low levels of Calcium ions which make the color change at the equivalence point difficult to see. Calcium is usually measured to screen for or monitor diseases of the bone or Calcium regulation disorders (that is, diseases of the parathyroid gland or kidneys).
Ultraviolet/visible (UV/vis) absorption spectroscopy is a technique that can be used to determine low levels of calcium. Because aqueous solutions of calcium salts are colorless and therefore do not
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MOLECULAR AND ATOMIC SPECTROSCOPY, Spectroscopy, Ultraviolet, Visible, Ultra-violet Visible Spectroscopy, Ultraviolet/visible spectroscopy Visible, 2 ULTRAVIOLET-VISIBLE ABSORPTION, 2 ULTRAVIOLET-VISIBLE ABSORPTION SPECTROSCOPY, Ultraviolet - Visible Spectroscopy, Visible Spectroscopy, EXPERIMENT 2: INTRODUCTION TO SPECTROSCOPY, Ultraviolet-Visible Spectroscopy, Spectroscopy 2 Ultraviolet-Visible Spectroscopy, ABSORPTION SPECTROPHOTOMETRY, ULTRAVIOLET, ABSORPTION SPECTROPHOTOMETRY, ULTRAVIOLET AND VISIBLE