Transcription of 2.2.25. ABSORPTION SPECTROPHOTOMETRY, ULTRAVIOLET …
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2.2.25. ABSORPTION SPECTROPHOTOMETRY, ULTRAVIOLET …
EUROPEAN PHARMACOPOEIA ABSORPTION spectrophotometry , ULTRAVIOLET and visible07/2005 ABSORPTIONSPECTROPHOTOMETRY, ULTRAVIOLET AND VISIBLED etermination of (A)ofa solution is defined as the logarithm to base 10 of thereciprocal of the transmittance (T) for monochromaticradiation:T=I/I0,I0=intensi ty of incident monochromatic radiation,I=intensity of transmitted monochromatic the absence of other physico-chemical factors, theabsorbance (A) is proportional to the path length (b)throughwhich the radiation passes and to the concentration (c)ofthe substance in solution in accordance with the equation: =molar absorptivity, ifbis expressed in centimetresandcin moles per expressionrepresenting the specificabsorbance of a dissolved substance refers to the absorbanceof a 10 g/l solution in a 1 cm cell and measured at a definedwavelength so that:Unless otherwise prescribed, measure the absorbance at theprescribed wavelength using a path length of 1 cm and at20 1 ,themeasurementsare carried out with reference to the same solvent or thesame mixture of solvents.
Absorption spectrophotometry, ultraviolet and visible 07/2005:20225 2.2.25. ABSORPTION SPECTROPHOTOMETRY, ULTRAVIOLET AND VISIBLE Determination of absorbance.Theabsorbance(A)of a solution is defined as the logarithm to base 10 of the reciprocal of the transmittance (T) for monochromatic
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