Transcription of 2.2.32. LOSS ON DRYING - uspbpep.com
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2.2.32. LOSS ON DRYING - uspbpep.com
EUROPEAN PHARMACOPOEIA Loss on dryingusing the conditions recommended by the manufacturerof the equipment. Manufacturers of SDS-PAGE equipmentmay provide gels of different surface area and running time and current/voltage may needto vary as described by the manufacturer of the apparatusin order to achieve optimum separation. Check that the bottom of the gel, stop the the gel assembly from the apparatus and separatethe glass plates. Remove the spacers, cut off and discard thestacking gel and immediately proceed with OF PROTEINS IN GELSC oomassie staining is the most common protein stainingmethod with a detection level of the order of 1 g to 10 gof protein per band. Silver staining is the most sensitivemethod for staining proteins in gels and a band containing10 ng to 100 ng can be of the steps in gel staining are done at room temperaturewith gentle shaking ( on an orbital shaker platform)in any convenient container.
EUROPEAN PHARMACOPOEIA 6.0 2.2.32. Loss on drying using the conditions recommended by the manufacturer of the equipment. Manufacturers of SDS …
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