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High-performance liquid chromatography/mass …

LCMS\TBv31of 2 Royal Society of Chemistry 2008 ISSN 1757-5958amc technical briefsEditor: Michael ThompsonAnalytical Methods CommitteeAMCTB34 November 2008 High-performance liquid chromatography / mass spectrometry (LC/MS) mass spectrometry (MS) provides us with uniquelyvaluable information: molecular mass (via mass tocharge(m/z)ratio); molecular structural informationand quantitative data, all at high ,it is best to apply separation techniques to complexmixtures before mass spectrometry is liquid chromatography (LC) isexcellentfor separating mixtures but generally poor atidentification of compounds. Thecombination of thesetwo techniques (LC/MS)thusprovides the marriage of MS and LC was uneasy and manyinterfaceshave beentried over the last 20 the relationship is more harmonious,thanks tothe development of electrospray ionisation, the best meanscurrently available for transferring molecules from solutioninto the gas phase for MS InstrumentationFigur

The type of mass analyser defines the mass spectrometer. Thus there are sector instruments relying on magnetic and electric fields for m/z ion separation, quadrupole

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