Transcription of HPLC Column Selection Guide - Qmx
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HPLC Column Selection Guide - Qmx
hplc Column Selection Guide Column Parameters Internal Diameter An important parameter of a hplc Column is the internal diameter (ID) as this directly influences the detection sensitivity, selectivity of separation and the quantity of analyte that can be loaded onto the Column . Low ID columns have higher sensitivity and lower solvent consumption for analytical purposes but require low loading of sample Analytical scale columns ( and ) are the most common type of columns and often use a UV Vis absorbance detector Narrow bore columns with ID of are used for sensitive analytical applications and use more advanced UV Vis, fluorescence or mass detectors Large ID columns ( ) have a high loading capacity and so are used to purify usable amounts of sample material for preparative or semi preparative applications Particle size The size of the particles (beads)
HPLC Column Selection Guide Column Parameters Internal Diameter An important parameter of a HPLC column is the internal diameter (ID) as this directly influences the
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