Transcription of Immunohistochemistry Application Guide - Abcam
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Immunohistochemistry Application Guide - Abcam
1 Immunohistochemistry Application GuideCommon abbreviationsABC: Avidin biotin complexAEC: 3-amino-9-ethylcarbazoleAP: Alkaline phosphataseBCIP: 5-bromo-4-chloro-3-indolyl phosphateBSA: Bovine serum albuminDAB: 3,3 diaminobenzidineFISH: Fluorescence in situ hybridizationFITC: Fluorescein isothiocyanateFFPE: Formalin-fixed, paraffin embeddedHIER: Heat induced epitope retrievalHRP: Horseradish peroxidaseH2O2: Hydrogen peroxideICC: ImmunocytochemistryIHC: ImmunohistochemistryIP: ImmunoprecipitationISH: In situ hybridizationLSAB: Labeled steptavidin biotinKO: Knock outNBF: Neutral buffered formalinNBT: p-nitroblue tetrazolium chloridePBS: Phosphate buffered salinePFA: ParaformaldehydePIER: Proteolytic induced epitope retrievalRabMAb: Rab
embedding in paraffin . Fixation is achie ved by perfusion or immersion immediately following dissection . The process typically takes 4 - 24 hours .
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