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Immunohistochemistry (IHC)

Learn more | more | (IHC) Handbook Variables Influencing Experimental Design VariablesFactors*TissueSpecies, Type, Section Size and ThicknessTargetAbundance and Subcellular LocalizationEpitopeConformation Dependent Availability and Post-translational ModificationsSample PreparationParaffin-embedded or FrozenFixation MethodPerfusion or ImmersionFixativeAldehydes (Crosslinking), Alcohols (Precipitating), or AcetoneBlocking BufferNormal Serum, BSA, Casein, Various Detergents, and Salt ConcentrationsAntigen Retrieval MethodNone, Heat-induced Epitope Retrieval (HIER), and Protease-induced Epitope Retrieval (PIER)PermeabilizationTriton-X 100, Tween-20, or SaponinDetection MethodDirect, Indirect, or Signal Amplification MethodsPrimary AntibodyMonoclonal or PolyclonalSecondary AntibodySpecies Reactivity and LabelMultiplexSimultaneous or Sequential Antibody Addition, and Antibody SpeciesImaging MethodFluorescence or ChromogenicLabelFluorochromes (numerous options)Chromogenic Reagents: Enzymes (HRP, AP) and Sub

Chromogenic Staining Protocol IHC Workflow Troubleshooting Guide No or Poor Signal High Background Poor Tissue Morphology Uneven or Non-specific Staining Reference Buffers Bio-Techne Support Products for IHC 1 2-5 6-7 8-9 10-11 12-13 14 15-17 18-19 20-22 23 24 TABLE OF CONTENTS. Learn more novusbiocom

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Immunocytochemistry (ICC)

images.novusbio.com

amino acid containing components including flavin coenzymes (FAD, FMN) and pyridine nucleotides (NADH). Refer to Figure 4A for an example of autofluorescence emitted by Lipofuscin, a pigment that accumulates with age in many tissue types. Lipofuscin has autofluorescence properties that overlap with the emission spectra of commonly used ...

  Acid, Immunocytochemistry

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