Transcription of Introduction to Fluorescence Microscopy
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Introduction to Fluorescence Microscopy
Introduction to Fluorescence Microscopy Kelly Cude with revisions and additions by Kelly Burke What is Fluorescence ? Fluorescence results when molecules called fluorophores absorb light, which briefly raises their energy level to an excited state. They emit fluorescent light as they decay from this excited state (known as Stoke s Shift). In general, a fluorophore will be excited by high frequency light (wavelengths in the ultraviolet, violet, or blue region of the spectrum), and emit light at slightly lower frequencies (wavelengths in the green or red region of the spectrum). See Figure 1. Figure 1 Stokes Shift Excitation and Emission Profiles Fluorophores come in a range of colors that span the visible spectrum (traditionally red, green, and blue fluorophores are used).
Introduction to Fluorescence Microscopy . Kelly Cude with revisions and additions by Kelly Burke . What is Fluorescence? Fluorescence results when molecules called fluorophores absorb light, which briefly raises their energy level to an excited state.
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Introduction to Fluorescence, Introduction, Fluorescence Microscopy, Introduction to Fluorescence Microscopy, Fluorescence, Fluorescence Imaging, Introduction Fluorescence, Lecture Notes: Introduction to Spectroscopy, Introduction to Fluorescence and Phosphorescence, Introduction Fluorescence Microscopy, X-Ray Fluorescence