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Introduction to Single-cell RNA-Seq

Introduction to Single-cell RNA-SeqWally the Welsh CorgiConnecting & Computer PreliminariesMake sure your workshopprovided computer isconnected to the Broad or Broad Internal wireless do notconnect your personal items(laptop, phone, etc.) to these wireless networks; it will tax the wireless system and make the workshop less password for computers is password . Introduction to Single-cell RNA-SeqTimothy TickleBrian HaasAsmaBankapurCenter for Cell Circuits Computational Genomics Workshop 2017We Know Tissues are HeterogeneousCell Identity is More Than HistopathologyA cell participates inmultiple cell factors shape a cell s identity-Membership in a taxonomyof cell types-Simultaneous time-dependent processes-Response to theenvironment-Spatial positioningBefore We Get Started Single-cell RNA-Seq (scRNA-Seq) analysis methodology is developing.

Introduction to Single-cell RNA-Seq ... •Filter all reagent with a 80 micron strainer before microfluidics. •Some purchased devices add a hydrophobic coating. –Can deteriorate (2 months at best). –Recoating does work (in-house). 10X: Massively Parallel Sequencing.

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