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Kirby-Bauer Disk Diffusion Susceptibility Test Protocol

Downloaded from byIP: : Wed, 29 Aug 2018 12:55:47 American Society for Microbiology 2016 1 Kirby-Bauer disk Diffusion Susceptibility Test Protocol | | Created: Tuesday, 08 December 2009 Author Jan Hudzicki Information History The publication on penicillin by Alexander Fleming in 1928 is a milestone in the history of medicine. As more antimicrobial compounds were discovered, it was predicted that infectious diseases would be eliminated through the use of these antimicrobials (7). Unfortunately, the development of bacterial resistance to these antimicrobials quickly diminished this optimism and resulted in the need for physicians to request the microbiology lab to test a patient s pathogen against various concentrations of a given antimicrobial to determine Susceptibility or resistance to that drug. The original method of determining Susceptibility to antimicrobials was based on broth dilution methods (5, 7), which although still the gold standard today, are time consuming to perform.

Institute Performance Standards for Antimicrobial Disk Susceptibility Tests: Approved Standards 9th Edition (3). RECIPE Sterile saline in 2-ml tubes 0.5 McFarland standard Wickerham card Mueller-Hinton agar plates, 100 mm or 150 mm Caliper or ruler Antibiotic disksb Forceps Antibiotic disk dispenser (optional) 18- to 24-ho Vortex

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