Transcription of Lipofectamine® RNAiMAX Reagent
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Protocol Pub. No. MAN0007825 RNAiMAX ReagentProtocol OutlineA. Plate cells so they will be 60-80% confluent at the time of Prepare RNA-lipid Add RNA-lipid complexes to RNAiMAX Transfection ProtocolSee page 2 to view a typical RNAiMAX transfection Amounts96-well24-well6-wellFinal siRNA used per well1 pmol5 pmol25 pmolFinal lipofectamine RNAiMAXused per LReverse Transfection of RNAiReverse transfection is faster to perform than forward transfection and is the method of choice for high-throughput transfection. Perform reverse transfection by preparing complexes inside the wells, and then adding cells and medium.
Title: Lipofectamine® RNAiMAX Reagent Author: Life Technologies Subject: Lipofectamine® RNAiMAX Transfection Reagent is a proprietary formulation for transfecting small RNAs \(e.g., siRNA, Silencer® Select siRNA, Stealth® RNAi, mirVana miRNA mimics and inhibitors\) into a wide range of eukaryotic cells.
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