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Microscopy I Light and Electron Microscopy

Microscopy ILight and Electron MicroscopyReplica of van Leeuwenhoek s(1632-1723) microscope constructed c. 1670. Moody Medical Library, Univ. Texas Medical Branch, Galveston, TXUse the information in this tutorial to supplement the visuals in lab and the information in Chapters 1, 8 and 9 in your lab manual .Replica of Culpepper tripod microscope built c. 1725 by Edmund Culpepper (1670-1738). Collection of Moody Medical Library, Univ. Tex., Galveston, TX. (Replica by Replica Rara Ltd. Antique Microscopes)ContentsI. Types of MicroscopyA. Light Microscopy1. Brightfield2. Darkfield3. Phase Contrast4. Polarizing5. DIC6. laser scanningB. Electron Microscopy1. Scanning2. TransmissionII. Specimen PreparationA. Light MicroscopyB. Scanning Electron MicroscopyC. Transmission Electron MicroscopyReplica of Marshall Microscope, c.

The electron beam is absorbed or deflected by the heavy metal stains and shadows are cast onto film or a phosphorescent plate (image is a shadow) at the bottom of the column. - 2-D image - reveals internal cell structure - high resolution, high magnification

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