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Optical Microscope; • Scanning Electron Microscope (SEM ...

Lecture 3 Brief Overview of Traditional Microscopes Optical Microscope ; Scanning Electron Microscope (SEM); Transmission Electron Microscope (TEM); Comparison with Scanning probe Microscope (SPM) General philosophy Human beings use two kinds of means to gauge objectives: Seeing --- through eyes (light) Optical Microscope electronic Microscope (higher resolution due to short wavelength); Touching --- through hands (probe) SPM. Converting wavelength (nm) to Electron energy (eV): (nm), for example, a 500 nm light corresponds to = eV Principles of an Optical Microscope Development of Microscope takes us back to 400 years ago Robert Hooke s "Micrographia" (1665) A hair knot Room dusts Red blood cell Flu virus Organic nanobelts with strong

• Electron wave is a unique medium that can be used in imaging. By accelerating the electrons into high energy beam (via high voltage), the wavelength thus created is far shorter than white light. For example, for an electron beam produced from a 20 kV gun, the wavelength is only 1240.7/20,000 (eV) = 0.06 nm = 0.6 Å,

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