Transcription of pPICZ A, B, and C
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pPICZ A, B, and C
User Manual pPICZ A, B, and C Pichia expression vectors for selection on Zeocin and purification of recombinant proteins Catalog no. V190-20 Rev. Date: 7 July 2010 Manual part no. 25-0148 MAN00000034 ii iiiTable of Contents Kit Contents and Storage ..iv Accessory Cloning into pPICZ A, B, and Pichia Expression in Zeocin ..19 Map and Features of pPICZ A, B, and Lithium Chloride Transformation Construction of In Vitro Technical Purchaser iv Kit Contents and Storage Contents The following components are included with Catalog no. V190 20. Note that the pPICZ expression vectors are supplied in suspension. Component Quantity Composition pPICZ A Expression Vector 20 g 40 l of g/ l vector in 10 mM Tris HCl, 1 mM EDTA, pH pPICZ B Expression Vector 20 g 40 l of g/ l vector in 10 mM Tris HCl, 1 mM EDTA, pH pPICZ C Expression Vector 20 g 40 l of g/ l vector in 10 mM Tris HCl, 1 mM EDTA, pH GS115/ pPICZ /lacZ Positive Control strain 1 stab -- Shipping/Storage The components included with Catalog
For more information, visit our web site at www.invitrogen.com or contact Technical Support (page 32). Product Quantity Catalog no. X-33 Pichia strain 1 stab C180-00 GS115 Pichia strain 1 stab C181-00 KM71H Pichia strain 1 stab C182-00 SMD1168H Pichia strain 1 stab C184-00 pPICZα A, B, and C 20 μg each V195-20
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