Transcription of pPICZ A, B, and C
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pPICZ A, B, and C
User Manual pPICZ A, B, and C Pichia expression vectors for selection on Zeocin and purification of secreted, recombinant proteins Cat. no. V195-20 Rev. Date: 7 July 2010 Manual part no. 25-0150 MAN0000035 ii iii Table of Contents Important Accessory Products ..vii Introduction ..1 Overview ..1 Cloning into pPICZ A, B, and Multiple Cloning Site of pPICZ Multiple Cloning Site of pPICZ Multiple Cloning Site of pPICZ Pichia Transformation ..9 Expression in Purification ..15 Appendix ..17 Recipes ..17 Zeocin ..19 pPICZ Vector ..21 Lithium Chloride Transformation Construction of In Vitro Technical Purchaser Notification ..34 iv v Important Information Contents 6 g of each of pPICZ A, B, and C vector in TE buffer, pH * (40 l at 150 ng/ l) *TE buffer, pH : 10 mM Tris-HCl, 1 mM EDTA, pH Shipping/Storage The vectors are shipped on wet ice and should be stored at 20 C.
PureLink™ HiPure Plasmid Miniprep Kit 25 preps 100 preps K2100–02 K2100–03 PureLink™ HiPure Plasmid Midiprep Kit 25 preps 50 preps K2100–04 K2100–05 S.N.A.P.™ Miniprep Kit 100 reactions K1900-01 S.N.A.P.™ Midiprep Kit 2 reactions K1910-01 S.N.A.P.™ Gel Purification Kit 25 reactions K1999-25 Detecting Fusion Protein
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