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Protein extraction from Tissues and Cultured Cells using ...

PROTOCOLE urope - Diagenode / / // North America - Diagenode Inc. / / extraction from Tissues and Cultured Cells using Bioruptor Standard & PlusIntroductionProtein extraction from Tissues and Cultured Cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or Protein purification. Efficient disruption and homogenization of animal Tissues and Cultured Cells are required to ensure high yields of proteins. Diagenode s Bioruptor uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize Tissues and Cultured Cells in just one step.

» We recommend using 100 µl of an appropriate lysis buffer per 1x10^6 cells. » For Western blotting, cells might be lysed directly in 1x Laemmli buffer. After sonication, centrifuge extract at 14,000 rpm for 15 min. Transfer the supernatant to a new tube and boil for 3 min. The supernatant can be used in Western blot.

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RNA extraction from tissue - PROTOCOL using Bioruptor ...

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PROTOCOL Europe - Diagenode s.a. / orders@diagenode.com / info@diagenode.com // North America - Diagenode Inc. / orders.na@diagenode.com / info.na@diagenode.com www.diagenode.com 1 RNA extraction from tissue - using Bioruptor® (Standard/Plus) and RNA extraction kit Introduction Isolation of intact RNA is essential for many techniques used in …

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