Red Blood Cell (RBC) Lysis Protocols
Lysis Buffer (Multi-species) is specially formulated for optimal lysis of RBC in peripheral blood. It has been validated to work on whole blood from human, mouse, rat, canine and non-human primate sources. The 1X RBC Lysis Buffer is optimized for lysis of RBC in human peripheral blood or single-cell suspensions of
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document:
Documents from same domain
Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com• Precipitation has an advantage over dialysis or desalting methods in that it enables concentration of the protein sample as well as purification from undesirable substances. • One disadvantage of protein precipitation is that proteins might denature, making the pellet difficult to re-solubilize.
Protein, Acetone, Precipitation, Acetone precipitation of proteins
Thermo Scienti˜ c TSQ Quantiva Triple-Stage Quadrupole ...
tools.thermofisher.comThermo Scienti˜ c TSQ Quantiva Triple-Stage Quadrupole Mass Spectrometer ... • Thermo Scientifi c ... New Zealand +64 9 980 6700 Norway +46 8 556 468 00 Russia/CIS +43 1 333 50 34 0 Singapore +65 6289 1190 Spain +34 914 845 965 Sweden +46 8 556 468 00 Switzerland +41 61 716 77 00
Ni-NTA Purification System - Thermo Fisher Scientific
tools.thermofisher.com7 Introduction Overview Introduction The Ni-NTA Purification System is designed for purification of 6xHis-tagged recombinant proteins expressed in bacteria, insect, and mammalian cells. The system is designed around the high affinity and selectivity of Ni-NTA Agarose
TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A
tools.thermofisher.comTRIzol™ Reagent Catalog Numbers 15596026 and 15596018 Doc. Part No. 15596026.PPS Pub. No. MAN0001271 Rev. A.0 WARNING! Read the Safety Data Sheets (SDSs) and follow the
Interpaion IEnPp fR nmpmeft n CfhRn Labware Chemical ...
tools.thermofisher.com286 www.thermoscientifi c.com Labwre Olafira nOPao ymreblo LsFoOrao LuapeDisEHLFbDabwriweFb CMT TTLy TL TM TT T/C T/ / ewFb TT na as H MnCM TM TTM Chemhical,l contaroAcSnlno#E N,o u u b u b b M M M b u u .
Interpretation of Nucleic Acid 260/280 Ratios
tools.thermofisher.comT123 – TECHNICAL BULLETIN NanoDrop Lite Interpretation of Nucleic Acid 260/280 Ratios T123– Rev 1/2012 Thermo Scientific NanoDrop Products Wilmington, Delaware USA Technical support:nanodrop.techsupport@thermofisher.com
Interpretation, Acid, Nucleic, Interpretation of nucleic acid 260
Corona CAD Charged Aerosol Detector - Thermo Fisher …
tools.thermofisher.com3 Corona CaD DeteCtor SpeCifiCationS* Operating Mode Charged Aerosol Detection Mobile Phase Flow Rate Up to 2 mL/min Full Scale Output Range 1 pA to 470 pA
GeneAmp PCR System 9700 - Thermo Fisher Scientific
tools.thermofisher.comIntroduction and Safety 1-1 Introduction and Safety 1 Overview About This Chapter This chapter provides information to help you safely operate the GeneAmp PCR System 9700. In This Chapter The following topics are covered in this chapter: Topics See Page About This Manual 1-2
alamarBlue TI read only - Thermo Fisher Scientific
tools.thermofisher.comPage 1 of 27 alamarBlue® Assay U.S. Patent No. 5,501,959 Indications for Use The alamarBlue® Assay is designed to measure quantitatively the proliferation of various human and animal cell lines, bacteria and fungi. The bioassay may also be used to establish relative
Installation Guide -Chromeleon 7 - Thermo Fisher Scientific
tools.thermofisher.comNote: Indicates information of special interest. Tip: Indicates information that will help you to use the software more efficiently. 1.3 Other Documents
Guide, Installation, Chromeleon, Installation guide chromeleon 7
Related documents
Peripheral Blood Mononuclear Cell (PBMC) Isolation and Red ...
www.kumc.eduRBC lysis buffer can easily and inexpensively be made: 10x RBC Lysis Buffer 90 g. NH 4 Cl (0.155M) 10 g. KHCO 3 (0.01M) 370 mg. EDTA (0.1mM) Dissolve in one liter of ddH 2 O Filter sterilize through .22 micron filter This solution can be stored at 4 C for months. Dilute 1:10 in ddH 2
1 Buffer Preparation - MD Anderson Cancer Center
www.mdanderson.orgCell Lysis Buffer (Flag-IP buffer) (50 mM TrisHCl pH7.4, 250 mM NaCl, 0.5% Triton X100, 10% glycerol, 1 mM DTT, PMSF, PI (Roche)) 1L 50 ml 1 M Tris 7.4, 62.5 ml 4 M NaCl, 5 ml Triton X-100, 1 ml 1 M DTT, 100 ml glycerol. ChIP Solutions ChIP Sweeling buffer Stock Vol for 500 ml 5 mM PIPES pH 8.0 0.5 M 5 ml ...
Center, Preparation, Cancer, Buffer, Anderson, Lysis, Md anderson cancer center, Buffer preparation, Lysis buffer
Sample preparation for western blot - Abcam
docs.abcam.com3 Lysis buffer recipes NP-40 buffer – 150 mM sodium chloride – 1.0% NP-40 (Triton X 100 can be substituted for NP 40) – 50 mM Tris pH 8.0 This is a popular buffer for studying proteins that are cytoplasmic or membrane-
RIPA Buffer (10X) - Cell Signaling Technology
media.cellsignal.comlysis buffer without any difficulties. 4. Aggregation may be present in this buffer upon arrival due to the high concentrations of reagents included at the supplied 10X formulation. At times, the aggrega-tion persists despite warming to room temperature. …
Plasmid Isolation (Alkaline Lysis) - G-Biosciences
www.gbiosciences.comAdd 200 µ l Lysis Buffer and mix the contents by gently inverting the tube 4-5 times. This solution contains sodium hydroxide and SDS (sodium dodecyl sulfate). The sodium hydroxide denatures the plasmid and chromosomal DNA into single strands. SDS, an ionic (charged) detergent dissolv es the phospholipids in the membrane
Isolation, Alkaline, Buffer, Plasmid, Lysis, Plasmid isolation, Alkaline lysis, Lysis buffer
Protein extraction from Tissues and Cultured Cells using ...
www.diagenode.com» We recommend using 100 µl of an appropriate lysis buffer per 1x10^6 cells. » For Western blotting, cells might be lysed directly in 1x Laemmli buffer. After sonication, centrifuge extract at 14,000 rpm for 15 min. Transfer the supernatant to a new tube and boil for 3 min. The supernatant can be used in Western blot.
Form, Tissue, Protein, Buffer, Extraction, Cultured, Lysis, Lysis buffer, Protein extraction from tissues and cultured
DNA Plasmid Isolation Using Alkaline Lysis Method
2015.igem.org5. Add 150 µL resuspension buffer, resuspend the bacterial pellet properly by vortexing. 6. Add 200 µL lysis solution to bacterial suspension (freshly made), close the tube tightly and mix contents thoroughly by inverting the tube 4-6 times until the solution becomes viscious. 7.