Transcription of Protocol 3: Immunofluorescence on Frozen Sections
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Protocol 3: Immunofluorescence on Frozen Sections
Protocol 3: Immunofluorescence on Frozen Sections Brigitte Arduini, version 1, 2015-Mar-23 Modified from Manipulating the Mouse Embryo 3rd edition (2003). Nagy, Gertsenstein, Vintersten and Behringer, ed. Materials Phosphate Buffered Saline (PBS, 1X) Paraformaldehyde (PFA, 4%) see Support Protocol 1 Microscope slides, pre-coated Optimal Cutting Temperature (OCT) Compound 30% sucrose in PBS Cryotome blades (disposable or permanent depending on cryostat) Reagent scoop or slotted spoon Aluminum Foil Conical tube or screw-cap vial Pencil Slide box Humidity box (covered tray with dowels fixed to the bottom) Staining jars/slide holders Cover glass no.
Protocol 3: Immunofluorescence on Frozen Sections . Brigitte Arduini ,version 1, 2015 -Mar-23 . Modified from Manipulating the Mouse Embryo 3. rd. edition (2003). Nagy, Gertsenstein, Vintersten and Behringer, ed. Materials Phosphate Buffered Saline (PBS, 1X) Paraformaldehyde (PFA, 4%) – see Support Protocol 1 . Microscope slides, pre -coated
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