Transcription of Real-time PCR handbook - Gene-Quantification
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Real-time PCR handbook - Gene-Quantification
Real-time PCR handbookThe image on this cover is of an OpenArray plate which is primarily used for mid-density Real-time PCR on the QuantStudio 12K Flex system. The figure above shows the commonly used formats for Real-time assays96- and 384-well plates384-well TaqMan Array cardsOpenArray plates162345 Basics of Real-time PCRD igital PCRE xperimental designPlate preparationData analysisTroubleshootingContentsBasics of Real-time Basics of Real-time PCR1 Introduction 3 Overview of Real-time PCR 4 Overview of Real-time PCR and Real-time PCR components 5 Real-time PCR analysis terminology 7 Real-time PCR fluorescence detection systems 11 melting curve analysis 15 Use of passive reference dyes 16 Contamination prevention 17 Multiplex Real-time PCR 17 Internal controls and reference genes 19 Real-time PCR instrument calibration 203 Basics of Real-time PCR1 For Research Use Only.
content is high. 2. Annealing: During annealing, complementary sequences have an opportunity to hybridize, so an appropriate temperature is used that is based on the calculated melting temperature (T m) of the primers (5°C below the T m of the primer). 3. Extension: At 70-72°C, the activity of the DNA polymerase is optimal, and primer ...
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