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SELECTIVE AND DIFFERENTIAL MEDIA

Microbiology Laboratory (BIOL 3702L) Page 1 of 7. SELECTIVE AND DIFFERENTIAL MEDIA . Principle and Purpose Microorganisms are generally studied after being grown on or in a microbiological medium. There is an expansive variety of useful MEDIA for this purpose including many types of general growth MEDIA that support a wide spectrum of organims. Examples include defined MEDIA , such as glucose-salts, as well as rich, chemically complex MEDIA such as brain heart infusion agar and tryptic soy broth. However, these general growth MEDIA may permit the growth unwanted microorganisms from clinical or environmental samples that are not relevant to the purpose of the microbiological examination. To help resolve this issue, the components of differnt MEDIA are sometimes adjusted to enhance the growth of particular organisms or, alternatively, to retard the growth of unwanted microbes. In the clinical laboratory, this ability to preferentially grow one type of microbe over another is critical in determining the cause of an infection and perhaps the means by which to treat the disease.

identification of enteric organisms and is also used in the isolation of pathogens from foods and coliforms in water samples. Like HE agar, MAC incorporates bile salts, in addition to sodium chloride, which inhibits the growth of most Gram-positive bacteria. Differentiation of enteric microorganisms is achieved by the fermentation of lactose

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