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SELECTIVE AND DIFFERENTIAL MEDIA

49 SELECTIVE AND DIFFERENTIAL MEDIAS elective and DIFFERENTIAL MEDIA are used to isolate or identify particularorganisms. SELECTIVE MEDIA allow certain types of organisms to grow, and inhibitthe growth of other organisms. The selectivity is accomplished in several example, organisms that can utilize a given sugar are easily screened bymaking that sugar the only carbon source in the medium. On the other hand, SELECTIVE inhibition of some types of microorganisms can be achieved by addingdyes, antibiotics, salts or specific inhibitors which affect the metabolism orenzyme systems of the organisms.

Cultures of: Staphylococcus aureus Staphylococcus epidermidis Escherichia coli Enterobacter aerogenes Salmonella enteritidis 2. Petri dishes of Mannitol Salt agar, EMB agar, MacConkey’s agar and nutrient agar Procedure: (work in pairs) 1. Divide each plate of MSA and MacConkey’s agar into two sections. Divide the EMB agar plate into three ...

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  Coils, Staphylococcus, Escherichia, Aureus, Escherichia coli, Staphylococcus aureus staphylococcus

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