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Kirby-Bauer Disk Diffusion Susceptibility Test Protocol

Downloaded from byIP: : Wed, 29 Aug 2018 12:55:47 American Society for Microbiology 2016 1 Kirby-Bauer disk Diffusion Susceptibility Test Protocol | | Created: Tuesday, 08 December 2009 Author Jan Hudzicki Information History The publication on penicillin by Alexander Fleming in 1928 is a milestone in the history of medicine. As more antimicrobial compounds were discovered, it was predicted that infectious diseases would be eliminated through the use of these antimicrobials (7). Unfortunately, the development of bacterial resistance to these antimicrobials quickly diminished this optimism and resulted in the need for physicians to request the microbiology lab to test a patient s pathogen against various concentrations of a given antimicrobial to determine Susceptibility or resistance to that drug.

are Staphylococcus aureus ATCC 25923 (Biosafety level (BSL) 2), Escherichia coli ATCC 25922 (BSL 1), and Pseudomonas aeruginosa ATCC 27853 (BSL 2) (www.atcc.org), as the zone of inhibition for these organisms is known. Because the zone sizes are known for these organisms, they are recommended for use in the educational

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