Transcription of Sucrose Gradient Separation Protocol - MitoSciences
{{id}} {{{paragraph}}}
Example: bachelor of science
Sucrose Gradient Separation Protocol - MitoSciences
Sucrose Gradient Separation Protocol January 2007. CONTENT. I. REQUIRED REAGENTS AND EQUIPMENT 3. II. SAMPLE PREPARATION 4. III. SAMPLE SOLUBILIZATION 5. IV. Sucrose Gradient PREPARATION 6. V. CENTRIFUGATION OF GRADIENTS AND ELUCTION OF FRACTIONS 8. VI. SAMPLE ANALYSIS 9. VII. BUFFER RECIPES 10. VIII. OPTIMIZATION STEPS AND GENERAL TIPS 11. IX. TROUBLESHOOTING GUIDE 13. X. FLOW CHART 14. XI. NOTES 15. 2. I. REQUIRED REAGENTS AND EQUIPMENT. Reagents: n-dodecyl- -D-maltopyranoside (Lauryl maltoside; MitoSciences MS910). Phosphate buffered saline, PBS (section VII). Sucrose solutions 15, 20, 25, , 30 and 35 %. Double distilled water Protease inhibitor cocktail (section VII). 13 x 51 mm polyallomer centrifuge tubes (Beckman 326819). Equipment: Swing-out compatible ultracentrifuge and rotor ( Beckman ). pH meter, weighing balance and other standard lab equipment Laboratory benchtop microfuge Protein electrophoresis and Western blotting equipment 3.
4 II. SAMPLE PREPARATION The MitoSciences sucrose gradient separation procedure is a protein subfractionation method optimized for mitochondria.
Loading..
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document:
Related search queries
Continuous Flow Ultracentrifuge System for, Continuous Flow Ultracentrifuge System for Production, CENTIFUGES AND, Ultracentrifuge, ELECTROPHORESIS PROCEDURE HELENA, Cellular Therapies – Program Chairs, Centrifugation, Gradient Appendix, Appendix, Of formaldehyde and binary ethyleneimine, Of formaldehyde and binary ethyleneimine BEI