Transcription of Chapter 3 Centrifugation - Sinica
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Chapter 3 Centrifugation - Sinica
Chapter 3 Centrifugation Biochemistry and Molecular Biology (BMB). Introduction Basic Principle of sedimentation Types, care and safety of centrifuges Preparative Centrifugation Analytical Centrifugation Analytical Biochemistry (AB). Ultracentrifugation Koolman, Color Atlas of Biochemistry, 2nd edition 1. General Steps in Biochemical Separation 2. Separation of Macromolecules Chromatography, precipitation Electrophoresis, ultracentrifugation 3. Densities of biological material Material Density (g/cm3). Microbial cells - Mammalian cells - Organelles - Proteins DNA RNA 4. Introduction (MBM ). Principles of Centrifugation A centrifuge is a device for separating particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed In a solution, particles whose density is higher than that of the solvent sink (sediment), and particles that are lighter than it float to the top.
5 Introduction (MBM 3.1) Principles of centrifugation In a solution, particles whose density is higher than that of the solvent sink (sediment), and particles that are lighter than it float to the top.
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