Transcription of TaqMan Universal PCR Master Mix - Applied …
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DRAFTA pril 4, 2002 9:53 am, q M a n Universal PCR Master MixProtocolDRAFTA pril 4, 2002 9:53 am, Copyright 2002, 2010 Applied Biosystems. All rights Research Use Only. Not for use in diagnostic in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this use of uracil-N-glycosylase for carryover prevention is licensed by Life Technologies, Inc. under patentsand foreign equivalents for research purposes only. No right for use in other applications, including the diagnosis of disease in humans, anim-als, or plants under any patents owned by Life Technologies, Inc.
Introduction 1-3 Note The forklike-structure-dependent, polymerization-associated, 5´ to 3´ nuclease activity of AmpliTaq Gold DNA Polymerase during PCR. When the probe is intact, the proximity of the reporter dye to the
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