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Technical Note: An Introduction to Fluorescence …

998-0050 Revision A Page 1 of 15 Technical Note: An Introduction to Fluorescence Measurements Fluorescence is the molecular absorption of light energy at one wavelength and its nearly instantaneous re-emission at another, usually longer, wavelength. Some molecules fluoresce naturally and others can be modified to make fluorescent compounds. Fluorescent compounds have two characteristic spectra: an excitation spectrum (the wavelength and amount of light absorbed) and an emission spectrum (the wavelength and amount of light emitted). These spectra are often referred to as a compound's Fluorescence signature or fingerprint. No two compounds have the same Fluorescence signature. It is this principle that makes fluorometry a highly specific analytical technique. Fluorometry is the measurement of Fluorescence .

An Introduction to Fluorescence Measurements Fluorescence is the molecular absorption of light energy at one wavelength and its nearly instantaneous re …

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