Transcription of Thermo Scientific Crosslinking Technical Handbook
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Thermo Scientific Crosslinking Technical Handbook
Thermo Scientific Crosslinking Technical Handbook Protein 1 NH2. O NH. HN. S. O. O. || SO 3 - ||. S S N Protein 1 + HO-N. H ||. O. Protein 2. easy molecular bonding Crosslinking technology Reactivity chemistries, applications and structure references table of contents Crosslinkers Technical Handbook Introduction 1 Applications with Crosslinkers 14 25. What is Crosslinking ? 1 Creating protein conjugates 14 16. Chemical Reactivity of Crosslinkers Protein immobilization and Modification Reagents 2 9 onto solid supports 17 18. Amine-reactive chemical groups 3 4 Creation of immunotoxins 19. Carboxylic acid reactive Label transfer 19 21. chemical groups 4 5. Structure determination Sulfhydryl-reactive chemical groups 5 6 with heavy/light crosslinker pairs 22 23. Carbonyl-reactive chemical groups 6 8 Metabolic labeling 24.
Aryl azide Hydroxyl (nonaqueous)-reactive-OH Isocyanate Azide-reactive -N 3 Phosphine Amine-reactive chemicalgroups Primary amines (–NH 2) exists at the N-terminus of each polypeptide chain (called the alpha-amine) and in the side chain of lysine (Lys, K) residues (called the epsilon-amine). Because of its positive charge at physiologic ...
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