Transcription of Thermo Scientific Crosslinking Technical Handbook
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Thermo Scientific Crosslinking Technical Handbook Protein 1 NH2. O NH. HN. S. O. O. || SO 3 - ||. S S N Protein 1 + HO-N. H ||. O. Protein 2. easy molecular bonding Crosslinking technology Reactivity chemistries, applications and structure references table of contents Crosslinkers Technical Handbook introduction 1 Applications with Crosslinkers 14 25. What is Crosslinking ? 1 Creating protein conjugates 14 16. Chemical Reactivity of Crosslinkers Protein immobilization and Modification Reagents 2 9 onto solid supports 17 18. Amine-reactive chemical groups 3 4 Creation of immunotoxins 19. Carboxylic acid reactive Label transfer 19 21. chemical groups 4 5. Structure determination Sulfhydryl-reactive chemical groups 5 6 with heavy/light crosslinker pairs 22 23.
Introduction 1 What is crosslinking? 1 Chemical Reactivity of Crosslinkers ... the core tools bioconjugation. 3 Table 1. Popular crosslinker reactive groups for protein conjugation. ... crosslinkers and labeling compounds react with primary amines in slightly alkaline conditions to yield stable amide bonds. The reaction releases N ...
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