Transcription of TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A
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TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A
USER Guide . TRIzol Reagent Catalog Numbers 15596026 and 15596018. Doc. Part No. Pub. No. MAN0001271 Rev. WARNING! Read the Safety Data Sheets (SDSs) and follow the Required materials not supplied handling instructions. Wear appropriate protective eyewear, Unless otherwise indicated, all materials are available through clothing, and gloves. Safety Data Sheets (SDSs) are available MLS: Fisher Scientific ( ) or from other major laboratory supplier. Table 1 Materials required for RNA, DNA, and protein isolation Product information Invitrogen TRIzol Reagent is a ready-to-use Reagent , designed to Item Source isolate high quality total RNA (as well as DNA and proteins) from cell Equipment and tissue samples of human, animal, plant, yeast, or bacterial origin, Centrifuge and rotor capable of reaching MLS.
Isolate DNA Isolate DNA from the interphase and the lower phenol-chloroform phase saved from “Lyse samples and separate phases“ on page 2. a. Remove any remaining aqueous phase overlying the interphase. This is critical for the quality of the isolated DNA. b. Add 0.3 mL of 100% ethanol per 1 mL of TRIzol™ Reagent used for lysis.
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