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Using BD FACSDiva™ CST to Evaluate Cytometer …
Using BD FACSDiva CST To Evaluate Cytometer Performance,Create Custom assay Settings and Implement Cross-Instrument and Cross-Site Standardization of AssaysPART 1 Using BD FACSDivaUsing BD FACSDiva CST To CST To Evaluate Cytometer Performance, Evaluate Cytometer Performance,Create Custom assay Settings Create Custom assay Settings and and Implement CrossImplement Cross--Instrument and Instrument and CrossCross--Site Standardization of AssaysSite Standardization of AssaysPART 1 PART 1 Alan M. StallDirector, Advanced Cytometry TechnologiesBD BiosciencesAlan M. StallDirector, Advanced Cytometry TechnologiesBD BiosciencesAgenda: Designing and Performing a Multi-color assay Across Sites First principles Resolution sensitivity Qr: Fluorescence detection efficiency Br: Background noise Choosing gain settings (MFI) Taking into account differences amongindividual instruments Electronic Noise (SDen) Linearity How does CST chose gain settings. Setting Baseline Gains Baseline MFI Target Values Reset Target ValuesFor Research Use Only.
Using BD FACSDiva™ CST To Evaluate Cytometer Performance, Create Custom Assay Settings and Implement Cross-Instrument and Cross-Site Standardization of Assays
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