Transcription of Yeast Protocols Handbook
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Yeast Protocols Handbook
Yeast Protocols Handbook FOR RESEARCH USE ONLYPT3024-1 (PR742227)Published 11 February 2008 Yeast Protocols Handbook Clontech Laboratories, Inc. Protocol No. PT3024-1 2 Version No. PR742227 I. Introduction 4 II. Introduction to Yeast Promoters 5 III. Culturing and Handling Yeast 10 IV. Preparation of Yeast Protein Extracts 12 A. General Information 12 B. Preparation of Yeast Cultures for Protein Extraction 12 C. Preparation of Protein Extracts: Urea/SDS Method 13 D. Preparation of Protein Extracts: TCA Method 15 E. Troubleshooting 17 V.
In vivo Plate Assay Using X-gal in the Medium 25 C. Colony-lift Filter Assay 25 D. Liquid Culture Assay Using ONPG as Substrate 26 E. Liquid Culture Assay Using CPRG as Substrate 27 F. Liquid Culture Assay Using a Chemiluminescent Substrate 28 G. α-Gal Quantitative Assay 32 VII. Working with Yeast Plasmids 34 A. General Information 34
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