5.2 Protein purification
NTA) column. In a single step, this affinity matrix can purify a protein (starting concen-tration less than 1% of the total protein) to more than 95% homogeneity. Nitrilotriacetic acid (NTA) is a tetradentate chelating adsorbent developed by Roche Diagnostics GmbH. NTA occupies four of six ligand binding sites of the nickel ion, leav-
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