Direct ELISA protocol - Abcam
The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Blocking 4. Block the remaining protein-binding sites in the coated wells by adding 200 µl blocking buffer, 5% non fat dry milk/PBS, per well. Alternative blocking reagents include BlockACE or BSA. 5.
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