制限酵素を用いたプラスミド DNA の切断→Ligation …
1本振ったらブロックに戻し、もう1本を振るというように手早くやる。 ⑧1ml程度のGB-gel Solutionが2本ずつ出来るので、1回目700ul、2回目300ulという ように2回に分けてカラムを通し、全量を4℃、15,000rpmで遠心する（時間は1分弱、
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