DNA SEQUENCING – METHODS AND APPLICATIONS
DNA Sequencing – Methods and Applications 4 will permit sequencing of atleast 100 bases from the point of labelling. The purine specific reagent is dimethyl sulphate; and the pyrimidine specific reagent is hydrazine. In 1973 , Gilbert and Maxam reported the sequence of 24 base pairs using a method known as wandering- spot analysis.
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