Microbiome Analysis with QIIME2: A Hands-On Tutorial
Jun 21, 2018 · Earth Microbiome Project recommends 515f-806r primers, error-correcting barcodes • Not taking precautions to support amplicon sequencing Some Illumina machines require high PhiX, low cluster density • Selecting an inappropriate reference database E.g., Greengenes(16S) reference database when sequencing ITS
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