RT-PCR: Two-Step Protocol - MIT OpenCourseWare
2) Dissolve the oligonucleotide in 10mM Tris, pH7.5 to make a primer stock at 100µM concentration. 3) Dilute from this stock 1:20 (in water) to make a working solution at 5 µM for use in setting up PCR reactions. B) Setting up PCR reactions Negative Controls Use two negative controls among the PCRs. I.
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