Transcription of Lactate Assay Kit-WST - DOJINDO
1 Technical ManualLactate Assay Kit-WST 1 TCA 1) Lactate Assay Kit-WST WST mmol/l 96 1 Lactate Assay Kit-WST ( ) 0-5oC (450 nm ) 96 well (37oC) 20-200 l 1000 l 200 l 20 l Enzyme Solution 1 (n=3 ) Working solution Lactate standard solution Triton ( HP ) Dye Mixture stock solution Dye Mixture Reconstitution Buffer Dye Mixture stock solution Reconstitution Buffer (0-5oC) (4 ) Working solution (1) Dye Mixture stock solution Assay Buffer (2) (1) Enzyme Solution Working solution 1 Working solution Working solution 1 Working solution 1.
2 (Sample) (0 1 mmol/l) 1 well 20 l 2. Lactate standard solution 10 mmol/l Lactate Standard 50 l 450 l 1 mmol/l Lactate standard solution 2 (1, , , , , , , 0 mmol/l) ( 2 ) Lactate Lactate standard solution Triton 2 Lactate standard solution L256 : Lactate Assay Kit-WSTI ssued on December, 15, 20173. (1) Lactate standard solution Sample 20 l ( 3 ) 1 (n=3 ) (2) Working solution 80 l Working solution (3) 37oC 30 (4) 450 nm (5) (Sample) Lactate Lactate Lactate 3 Lactate standard solution (n=3) 4 Lactate 5 2-Deoxy-D-glucose 2-Deoxy-D-glucose (1) HeLa (1 104 cells/well 10% fetal bovine serum 1% penicillin-streptomycin MEM ) 96 well 37oC 5% CO2 (2) 2-Deoxy-D-glucose 100 l (3) 37oC 5% CO2 (4) ml 20 l 8 (5)
3 Lactate standard solution ( Lactate standard solution ) (6) Lactate standard solution 20 l 96 well (7) Working solution 80 l (8) 37oC 30 (9) 450 nm Lactate 2-Deoxy-D-glucose Lactate 1) , et al., Nature, 2017, 551, : Lactate Assay Kit-WST 2025-5 861-2202 Tel:096-286-1515 ( ) Fax:096-286-1525 E-mail: URL: ( ) 2-1-17 7F 105-0012 Tel: 03-3578-9651 ( ) Fax: 03-3578-9650 :0120-489548 :0120-021557
