10 Desalting

PD-10 Desalting Columns - GWDG

PD-10 Desalting Columns are prepacked and designed for rapid, convenient sample clean-up of proteins and other large biomolecules (>5000 M r). PD-10 Desalting Columns can be used in a wide range of applications such as desalting, buffer exchange and removal of low-molecular weight compounds.

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HisTrap HP, 1 ml and 5 ml - Auburn University

a HiTrap™ Desalting, a PD-10 Desalting Column, or HiPrep™ 26/10 Desalting, (see Table 4). Leakage of Ni2+ from Ni Sepharose High Performance is very low under all normal conditions, lower than for other IMAC media tested. For applications where extremely low leakage during purification is critical,

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Protein Concentration and Diafiltration by Tangential Flow ...

desalting, or concentration. Depending on the protein to be retained, membrane NMWLs in the range of 1 kD to 1000 kD are used. Two types of UF are Virus filtration (VF) and High Performance tangential flow filtration (HPTFF). For VF, membrane NMWL ratings range from 100 kD to 500 kD, or up to 0.05 µm. This process type is used to separate

  Desalting

Physical and Chemical Properties of Crude Oil and Oil Products

If the salt content of the crude, when expressed as NaCl, is greater than 10 lb/1000 bbl, it is generally necessary to desalt the crude before processing. If the salt is not removed, severe corrosion problems may be encountered. If residua are processed catalytically, desalting is desirable at even lower salt contents of he crude.

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DNA Clean & Concentrator®-5 - Zymo Research

Mar 17, 2021 · Efficient desalting of DNA with the removal of DNA polymerases, primers, and free dNTPs. DNA Clean-up From Enzymatic Reactions ... Add 10 µl 0.5 M EDTA and 10 µl 1 N NaOH to 50 µl of RT reaction. The volumes of EDTA and NaOH should be …

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Tutorial: Delayed Coking Fundamentals - Campus Tour

In desalting, crude oil is washed with around 5% water to remove the salts and dirt from the crude oil . The water, being heavier than the oil, drops out of the ... 10 mm Hg absolute desired but more common 25 to 100 mm Hg absolute. The desired ai m is to lift the maximum amount of oil boiling below 565 °C into

  Fundamentals, Tutorials, Delayed, Coking, Desalting, Delayed coking fundamentals

Thermo Scientific Crosslinking Technical Handbook

(MW 115g/mol), which can be removed easily by dialysis or desalting. NHS-reactive chemistry NHS ester crosslinking reactions are most commonly performed in phosphate, to four hours at room temperature or 4°C. Primary amine buffers such as Tris (TBS) are not compatible, because they compete for reaction. However in some

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Principles of MALDI-TOF mass spectrometry

Desalting Ziptip. 684.11 78 9.5 7 1048.72 1122.79 1165.80 1277.83 1461.00 1705.16 1838.26 1937.22 1994.24 2137.21 2284.25 2384.27 26 47.50 0 5000 10000. Counts. 15000 1000 1500 2000 2500 3000 . Mass (m/z) Applications of time-of-flight. Purdue-UAB Botanicals Center for Age-Related Disease ...

  Desalting

Acetone precipitation of proteins - Thermo Fisher Scientific

66380 Slide-A-Lyzer® Dialysis Cassette, 10 MWCO, 0.5-3ml, 10 units (see web site for a complete listing) 89865 2D Sample Prep for Soluble Protein, Sufficient reagents for 25 applications 23215 Compat-Able TM Protein Assay Preparation Reagent Set , sufficient reagents to pre-treat 500

DNA EXTRACTION METHODS - Open University of Sri Lanka

•TE Buffer - Tris-EDTA Buffer: 10 mM Tris-HCl pH 8.0, 1 mM EDTA, or TE-4 which is 10 mM Tris, 0.1 mM EDTA. DNA is resuspended and stored in TE buffer. DNA must be stored in a slightly basis buffer to prevent depurination, and the EDTA chelates any Mg2+ helping to inactivate DNases. •DNA can be stored at 4oC for extended periods,