Search results with tag "Desalting"
PD-10 Desalting column - Wolfson Centre Home Page
wolfson.huji.ac.il• p2 PD-10 Desalting Workmate and LabMate Buffer Reservoir PD-10 Desalting Workmate is designed to fulfil the needs of a column stand for PD-10 Desalting columns.
HisTrap HP, 1 ml and 5 ml - Auburn University
webhome.auburn.edua HiTrap™ Desalting, a PD-10 Desalting Column, or HiPrep™ 26/10 Desalting, (see Table 4). Leakage of Ni2+ from Ni Sepharose High Performance is very low under all normal conditions, lower than for other IMAC media tested. For applications where extremely low leakage during purification is critical,
DNA Clean & Concentrator®-5 - Zymo Research
files.zymoresearch.comMar 17, 2021 · Efficient desalting of DNA with the removal of DNA polymerases, primers, and free dNTPs. DNA Clean-up From Enzymatic Reactions Efficient desalting of DNA with the removal of modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, endonucleases, etc. Post-Reverse Transcription (RT) & cDNA Clean-up
The all-in-one” system for the inducible expression of shRNA
media.addgene.orgOn cohesive ends. Standard desalting is sufficient for oligo purification. Oligo annealing and ligation into pLKO-Tet-On: 1. Bring oligo powders to room temp and spin down for a few seconds. 2. Reconstitute oligos in ddH 2O to 0.1 nmol/µl : look at the amount of oligo printed on the tube and add the appropriate amount of ddH 2O (e.g. 21 nmol ...
P rotein preparation handbook - Thermo Fisher Scientific
assets.thermofisher.comZeba™ desalting products contain a unique resin and are specifically designed to provide consistent performance over a wide range of protein concentrations and sample sizes. High recovery of protein can be achieved even for dilute protein samples. • Concentration Protein concentration and diafiltration, similar to
Physical and Chemical Properties of Crude Oil and Oil Products
ceng.tu.edu.iqIf the salt content of the crude, when expressed as NaCl, is greater than 10 lb/1000 bbl, it is generally necessary to desalt the crude before processing. If the salt is not removed, severe corrosion problems may be encountered. If residua are processed catalytically, desalting is desirable at even lower salt contents of he crude.
Tutorial: Delayed Coking Fundamentals - Campus Tour
inside.mines.eduIn desalting, crude oil is washed with around 5% water to remove the salts and dirt from the crude oil . The water, being heavier than the oil, drops out of the ... 10 mm Hg absolute desired but more common 25 to 100 mm Hg absolute. The desired ai m is to lift the maximum amount of oil boiling below 565 °C into
Thermo Scientific Crosslinking Technical Handbook
tools.thermofisher.com(MW 115g/mol), which can be removed easily by dialysis or desalting. NHS-reactive chemistry NHS ester crosslinking reactions are most commonly performed in phosphate, to four hours at room temperature or 4°C. Primary amine buffers such as Tris (TBS) are not compatible, because they compete for reaction. However in some
Protein Concentration and Diafiltration by Tangential Flow ...
wolfson.huji.ac.ildesalting, or concentration. Depending on the protein to be retained, membrane NMWLs in the range of 1 kD to 1000 kD are used. Two types of UF are Virus filtration (VF) and High Performance tangential flow filtration (HPTFF). For VF, membrane NMWL ratings range from 100 kD to 500 kD, or up to 0.05 µm. This process type is used to separate
DNA EXTRACTION METHODS - Open University of Sri Lanka
www.ou.ac.lk•Desalting and buffer exchange •Removal of primers, linkers and Dr.L.Yatawaraunincorporated label. Concentrating DNA Microcon®100 Centrifugal Filter Unit •Patented deadstop allows for reliable and reproducible concentrate volumes •Inverted spin method of concentrate
QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I)
schnablelab.plantgenomics.iastate.eduApr 01, 2007 · ordered from IDT. Primer purification: standard desalting. • SuperScript First-Strand Synthesis System for RT (Invitrogen). • SYBR Green PCR master mix (Applied Biosystems, cat log: 4309155). • 96-well plates (Stratagene, cat.: 410021) • 8-tube strip tubes (Stratagene, cat.: 410022) • Optical strip caps (Stratagene, cat.: 410024)
Principles of MALDI-TOF mass spectrometry
www.uab.eduDesalting Ziptip. 684.11 78 9.5 7 1048.72 1122.79 1165.80 1277.83 1461.00 1705.16 1838.26 1937.22 1994.24 2137.21 2284.25 2384.27 26 47.50 0 5000 10000. Counts. 15000 1000 1500 2000 2500 3000 . Mass (m/z) Applications of time-of-flight. Purdue-UAB Botanicals Center for Age-Related Disease ...
PD-10 Desalting Columns - GWDG
wwwuser.gwdg.deThe chromatography technique is gel filtration and molecules are separated on the basis of differences in size. ... Exclusion limit, M r Bed volume Sample volume gravity protocol 1 Sample volume spin protocol PD SpinTrap™ ... or other biomolecule. Typically the recovery is …