2x Laemmli Sample
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Product Information 2x Laemmli Sample 2x Laemmli Sample ...
www.bio-rad.com4x Laemmli sample buffer: Add 100 µl of 2-mercaptoethanol per 900 µl. Alternatively, add dithiothreitol (DTT or Cleland’s reagent) to a final 1x concentration of 50 mM. Note: For best results, do not store sample buffer with 2-mercaptoethanol. 2. Dilute Sample 2x Laemmli sample buffer: Dilute 1 part sample with 1 part 2x Laemmli sample buffer.
General western blot protocol - Abcam
docs.abcam.comDetermine how much protein to load and add an equal volume 2X Laemmli sample buffer. We recommend reducing and denaturing the samples using the following method unless the online antibody datasheet indicates that non-reducing and non-denaturing conditions should be used. 3. To reduce and denature your samples, boil each cell lysate in sample ...
General Protocol for Western Blotting - Bio-Rad …
www.bio-rad.com10. Take 20 μg of each sample and add an equal volume of 2x Laemmli sample buffer. 11. Boil each cell lysate in sample buffer at 95°C for 5 min. 12. Centrifuge at 16,000 x g in a microcentrifuge for 1 min. Bulletin 6376 Protocol General Protocol for Western Blotting
Protocol: Protein electrophoresis and western blot recipes
assets.thermofisher.comSDS sample buffer (Laemmli buffer): 63 mM Tris-HCl, 10% glycerol, 2% SDS, 0.0025% bromophenol blue, pH 6.8 Recipe for 2X buffer stock: 0.5 M Tris-HCl, pH 6.8 2.5 mL Glycerol 2 mL 10% (w/v) SDS 4 mL 0.1% (w/v) bromophenol blue 0.5 mL Deionized water to 10 mL The buffer is stable for 6 months when stored at 4°C. 10% SDS SDS 1.00 g
Sample preparation for western blot - Abcam
docs.abcam.comThe standard loading buffer is called 2X Laemmli buffer (Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bateriophage T4. Nature, 227, 680–5). It can also be made at 4X and 6X strength to minimize dilution of the samples. The 2X is to be mixed in 1:1 ratio with the sample. 2X Laemmli buffer recipe – 4% SDS