Search results with tag "Laemmli sample"
Product Information 2x Laemmli Sample 2x Laemmli Sample ...
www.bio-rad.com4x Laemmli sample buffer: Add 100 µl of 2-mercaptoethanol per 900 µl. Alternatively, add dithiothreitol (DTT or Cleland’s reagent) to a final 1x concentration of 50 mM. Note: For best results, do not store sample buffer with 2-mercaptoethanol. 2. Dilute Sample 2x Laemmli sample buffer: Dilute 1 part sample with 1 part 2x Laemmli sample buffer.
Product Information 2x Laemmli Sample 2x Laemmli Sample ...
www.bio-rad.comIntroduction Bio-Rad’s Laemmli sample buffers are based on the method of Laemmli (1970). The use of Laemmli sample buffers ensures optimal band resolution
General Protocol for Western Blotting - Bio-Rad …
www.bio-rad.com10. Take 20 μg of each sample and add an equal volume of 2x Laemmli sample buffer. 11. Boil each cell lysate in sample buffer at 95°C for 5 min. 12. Centrifuge at 16,000 x g in a microcentrifuge for 1 min. Bulletin 6376 Protocol General Protocol for Western Blotting
General western blot protocol - Abcam
docs.abcam.comSample preparation 1. Remove a small volume of lysate to perform a protein quantification assay. Determine the protein concentration for each cell lysate. 2. Determine how much protein to load and add an equal volume 2X Laemmli sample buffer. We recommend reducing and denaturing the samples using the following
General Protocol for Western Blotting - Bio-Rad
www.bio-rad.com10. Take 20 μg of each sample and add an equal volume of 2x Laemmli sample buffer. 11. Boil each cell lysate in sample buffer at 95°C for 5 min. 12. Centrifuge at 16,000 x g in a microcentrifuge for 1 min. Bulletin 6376 Protocol General Protocol for Western Blotting