Biuret
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Mark scheme (AS) : Paper 1 - June 2017 - AQA
filestore.aqa.org.uk05.1 Biuret; 1 Ignore any other detail Accept Copper sulfate and sodium hydroxide CuSO 4 + NaOH Alkaline copper sulfate Copper sulphate and sodium hydroxide Alkaline copper sulphate Biurette Buiret Biruet Bieuret Reject burette or Beirut 05.2 Draw around H 2 N – C – COOH; H 1 05.3 Nitrogen; 1 Ignore N
Exercise 21 - NCERT
ncert.nic.in(a) Biuret test • Take 2 mL of protein solution (milk, albumin of egg or gram seed extract) in a test tube. • Add 1 mL of 40% NaOH solution and 1 or 2 drops of 1% CuSO 4 solution. • A violet colour indicates the presence of proteins. Care must be taken that excess of copper sulphate is not added otherwise
Mark scheme (Higher) : Paper 1 - June 2019
filestore.aqa.org.ukaccept biuret 1 AO1 4.4.2.5 . 02.4 . 27.6 (cm. 3) allow 27.60 (cm. 3) 1 AO2 4.4.2.5 . MARK SCHEME – GCSE CHEMISTRY – 8462/1H – JUNE 2019 . 10 Question 2 continued . Question . Answers : Mark . AO/ Spec. Ref . 02.5 : Level 3: The design/plan would lead to the production of a valid outcome. All key steps are identified and logically sequenced.
タンパク定量 (BCA法)
www.iqb.u-tokyo.ac.jp一段階のBiuret反応でできるCu(I)とペプチドの錯体自体にも吸光はありますが、測定に使うには弱すぎます。 なので、二段階目のBCAとの錯体形成反応で562nmに吸収極大を持つより吸光度の高い錯体へと変換していま す。