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Found 10 free book(s)La Roche House Le Corbusier and Pierre Jeanneret. Photo ...
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www.bio-rad.com3 2.2 DNA Preparation From Cultured Mammalian Cells 1 Pellet 200 µl of cell suspension from media in a microfuge tube. Spin at 10,000–12,000 rpm for 1 minute and remove the supernatant. 2 Resuspend cells in 1 ml of 1x PBS and centrifuge for 1 minute at 10,000–12,000 rpm. 3 Resuspend cells in autoclaved water at 20–30 cells/µl. 4 Add 20 µl of this cell suspension to 200 µl of ...
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1.1 Les phases d’un projet minier - ELAW
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