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Red Blood Cell (RBC) Lysis Protocols
tools.thermofisher.com1X RBC Lysis Buffer is optimized for lysis of RBC in human peripheral blood or single-cell suspensions of mouse hematopoietic tissues such as spleen or bone marrow. General Notes Before use, the 10X RBC Lysis Buffer (Multi-species) must be diluted 1:10 with room temperature, reagent-grade water.
TRANSFECTION PROTOCOL Lipofectamine 3000 …
assets.thermofisher.comComponent Cat. No. Gibco™ Ham’s F-12 Nutrient Mix 11765054 5% Horse Serum – 1 mM Gibco™ Sodium Pyruvate 11360070 25 mM Gibco™ HEPES 15630106 Proper culture techniques and procedures are an essential part of ensuring successful transfection. Subculturing, also referred to as passaging, is the removal of medium and