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TRANSFECTION PROTOCOL Lipofectamine 3000 …

TRANSFECTION PROTOCOL Lipofectamine 3000

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Component Cat. No. Gibco™ Ham’s F-12 Nutrient Mix 11765054 5% Horse Serum – 1 mM Gibco™ Sodium Pyruvate 11360070 25 mM Gibco™ HEPES 15630106 Proper culture techniques and procedures are an essential part of ensuring successful transfection. Subculturing, also referred to as passaging, is the removal of medium and

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