Search results with tag "Lipofectamine"
Protocol: Lipofectamine 3000 Transfection Reagent HepG2 ...
assets.fishersci.comTransfection protocol Transfection components Component Cat. No. Thermo Scientific ™ Nunc 24-Well Cell-Culture Treated Multidishes 142475 Invitrogen ™ Lipofectamine 3000 Transfection Reagent L3000008 Gibco ™ Opti-MEM I Reduced Serum Medium 31985062 Step Tube Complexation components Amount per well (24-well)
TRANSFECTION PROTOCOL Lipofectamine 3000 …
assets.thermofisher.comComponent Cat. No. Gibco™ Ham’s F-12 Nutrient Mix 11765054 5% Horse Serum – 1 mM Gibco™ Sodium Pyruvate 11360070 25 mM Gibco™ HEPES 15630106 Proper culture techniques and procedures are an essential part of ensuring successful transfection. Subculturing, also referred to as passaging, is the removal of medium and
96-well 24-well 6-well - Thermo Fisher Scientific
tools.thermofisher.comLipofectamine ™ 3000 Reagent Protocol. Protocol Outline. A. Plate cells so they will be 70–90% confluent at the time of transfection. B. Prepare plasmid DNA-lipid complexes (recommend 2 doses of lipid). C. Add DNA-lipid complexes to cells. Transfection Amounts Transfection of siRNA. To transfect cells with siRNA, follow the protocol as ...
Lipofectamine® RNAiMAX Reagent - Thermo Fisher Scientific
tools.thermofisher.comSelection Guide Lipofectamine® Reagents Go online to view related products. Product Description ∤ Lipofectamine® RNAiMAX Transfection Reagent is a proprietary formulation for transfecting small RNAs (e.g., siRNA, Silencer ® Select siRNA, Stealth RNAi, mirVana™ miRNA mimics and inhibitors) into a wide range of eukaryotic cells. Important ...